Yiying Yang
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Journal of Advanced Transportation
and
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PUMILIO proteins maintain lineage balance via post-transcriptional repression of germline fate and promotion of somatic differentiation
Generation of Pum1 and/or Pum2 knockout mESCs Paired guide RNAs (gRNAs) targeting specific sequences within exon 12 of Pum1 and exon 6 of Pum2, with a spacing of 5–30 bp (Figure S1A), were designed using the Benchling-sgRNA online tool (https://www.benchling.com/crispr/). Each sgRNA pair was cloned into a pGL3-U6-sgRNA-PGK-puromycin vector (Addgene plasmid Cat# 51133).
Computer Science > Computer Vision and Pattern Recognition
Complex image creation and editing often require more than a single generation or editing model. A user request may involve synthesizing images, localizing objects, segmenting regions, editing selected content, compositing intermediate assets, reading text, and enhancing the final result.
Queen cell architecture shapes honey bee queen development
Abstract Nest architecture in social insects is often viewed as a static structural component providing shelter or storage1. However, the extent to which these constructed environments actively shape biological traits remains poorly understood.
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