Yu-Lu Zhang

Abstract Precise, site-specific insertion of large gene sequences holds great promise for the treatment of diverse genetic disorders. Although prime editing using paired guide RNAs (pegRNAs) can mediate targeted integration, insertion efficiency drops sharply for payloads exceeding 300 base pairs1,2,3. Here we present a rationally designed quadruple pegRNA strategy (QuadPE) for efficient and programmable insertion of large DNA fragments.

Abstract Kidney diseases, including acute kidney injury (AKI) and chronic kidney disease (CKD), are associated with changes in the composition and function of gut microbiota.

Dear Editor, Recent studies on endogenous circular RNAs (circRNAs) have revealed their high stability and unique conformation.1,2,3 Engineered in vitro circularized RNAs (ivcRNAs), which serve as advanced platforms for sustained protein expression,4,5 exhibit greater stability and lower immunogenicity than their linear counterparts.4,5,6,7,8 Because ivcRNAs lack a 5′ m⁷G cap structure, they typically rely on a structured internal ribosome entry site (IRES) to initiate cap-independent...