Yunsun Nam

Abstract Specific deposition of RNA modifications is important for regulating gene expression1,2. 5-Methylcytosine (m5C) is a common epitranscriptomic modification, and NSUN2 is a key enzyme responsible for m5C methylation of various types of RNA. Dysregulation of NSUN2 is associated with numerous diseases, including cancers and neurological disorders3. The versatility of NSUN2 complicates our understanding of its substrate specificity and molecular roles in biology and disease.

Published In Science Advances Volume 10 | Issue 51 December 2024 Article versions Submission history Received: 14 August 2024 Accepted: 14 November 2024 Permissions Request permissions for this article. Acknowledgments We thank O. Koczy, J. Parker, and R. Setlem for contributing to preliminary studies. We are grateful for the helpful discussions with B. Xhemalce on genomic data analysis. The Structural Biology Laboratory at UT Southwestern for help with synchrotron data collection.

Abstract MicroRNAs modulate most protein-coding genes, and many are regulated during maturation. Chemical modifications of primary transcripts containing microRNAs have been implicated in altering Microprocessor processing efficiency, a key initiating endonucleolytic step performed by Drosha and DGCR8.