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Zharko Daniloski on Muck Rack

Zharko Daniloski

As seen in: Cell Press, eLife
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Articles

The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types

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By Zharko Daniloski, Tristan X. Jordan, Xinyi Guo, Juliana K Ilmain
| eLife
Abstract A novel variant of the SARS-CoV-2 virus carrying a point mutation in the Spike protein (D614G) has recently emerged and rapidly surpassed others in prevalence. This mutation is in linkage disequilibrium with an ORF1b protein variant (P314L), making it difficult to discern the functional significance of the Spike D614G mutation from population genetics alone. Here, we perform site-directed mutagenesis on wild-type human codon optimized Spike to introduce the D614G variant.
Open in Who Shared Issue with article?

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells

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By Zharko Daniloski, Tristan X. Jordan, Hans-Hermann Wessels, Daisy Hoagland, Silva Kasela, Mateusz Legut, Silas Maniatis, Eleni P. Mimitou, Lu Lu, Oded Danziger, Brad Rosenberg, Peter Smibert, Tuuli Lappalainen, Evan Geller, Hemali Phatnani
| ScienceDirect Verified
Highlights • Genome-wide CRISPR knockout screen identifies host factors for SARS-CoV-2 infection • Top-ranked genes include vacuolar ATPases, Retromer, Commander, and Arp2/3 complex • Validation using CRISPR knockout, RNA interference, and small molecule inhibitors • Reduced infection via increased cholesterol biosynthesis and sequestration of ACE2 Summary To better understand host-virus genetic dependencies and find potential therapeutic targets for COVID-19, we performed a genome-scale...
Open in Who Shared Issue with article?

Identification of required host factors for SARS-CoV-2 infection in human cells

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By Zharko Daniloski, Tristan X. Jordan, Hans-Hermann Wessels, Daisy Hoagland
| Cell Press Verified
Highlights • Genome-wide CRISPR knockout screen identifies host factors for SARS-CoV-2 infection • Top-ranked genes include vacuolar ATPases, Retromer, Commander and Arp2/3 complex • Validation using CRISPR knockout, RNA interference and small molecule inhibitors • Reduced infection via increased cholesterol biosynthesis and sequestration of ACE2 Summary To better understand host-virus genetic dependencies and find potential therapeutic targets for COVID-19, we performed a genome-scale CRISPR...
Open in Who Shared Issue with article?
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