Adam Croft

Abstract Regulatory T cells (Treg cells), characterized by FOXP3 expression, maintain immune homeostasis, but their function is impaired in autoimmune diseases such as rheumatoid arthritis (RA). Here we used single-cell RNA sequencing to analyze Treg cells in synovial tissues from patients with RA. We identified two predominant Treg states, CD25hiCXCR6pos Treg cells and dysfunctional CD25loAREGpos Treg cells, both enriched in synovial tissues but not in blood.

Abstract Ablative fractional carbon dioxide (AFCO2) laser therapy is used for treating pathological scarring. However, mechanisms underlying reduction in hypertrophic scarring are poorly understood. We investigated cellular mechanisms of AFCO2 laser therapy by performing single-cell RNA sequencing (scRNA-seq) on skin biopsies from burn survivors with hypertrophic scars before and after three sessions of AFCO2 therapy. Scar reduction was assessed subjectively and objectively.