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Abstract The mammalian brain consists of diverse neuron types with various functions. Recent single-cell RNA sequencing approaches have led to a whole-brain taxonomy of transcriptomically defined cell types1. Patch-seq experiments augment these cell-type descriptions by linking transcriptomic profiles with local morphological and electrophysiological properties2,3,4,5,6,7. However, linking transcriptomic identities to long-range axonal projections remains a major unresolved challenge.
Keywords epigenetics transcriptomics enhancer cell type neocortex AAV transgenic recombinase mouse human Introduction To understand how the brain works, we need to establish the structure-function relationships between the units that comprise it and the outputs of its activity.1 One approach is to gain experimental access to each building block (e.g., a cell type) and manipulate it to observe effects on an animal’s physiology and behavior.2,3,4 Historically, the definition of building blocks...
First week of classes. Piles of syllabi. Hours spent in Olin Library. Navigating the chaos of the club fair. These past few days have brought on a whirlwind of emotions for the start of Washington University’s fall 2022 semester, especially for the incoming class of 2026.
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