Christopher H Yin

Keywords pre-mRNA 3′ end processing cleavage and polyadenylation nuclear speckle biomolecular condensate membraneless organelle phase separation gene expression intrinsically disordered region transcription termination Introduction The nucleus contains various membraneless organelles, biomolecular condensates formed by specific proteins and nucleic acids.1,2,3 One such organelle is the nuclear speckles (NSs), which are enriched with many splicing factors and RNA molecules.4,5 Some NS...

Keywords pre-mRNA 3′ end processing cleavage and polyadenylation nuclear speckle biomolecular condensate membraneless organelle phase separation gene expression intrinsically disordered region transcription termination Get full text access Log in, subscribe or purchase for full access. References 1. Belmont, A.S. Nuclear Compartments: An Incomplete Primer to Nuclear Compartments, Bodies, and Genome Organization Relative to Nuclear Architecture Cold Spring Harb. Perspect. Biol. 2022; 14, a041268 2.

Abstract An important and largely unsolved problem in synthetic biology is how to target gene expression to specific cell types. Here, we apply iterative deep learning to design synthetic enhancers with strong differential activity between two human cell lines. We initially train models on published datasets of enhancer activity and chromatin accessibility and use them to guide the design of synthetic enhancers that maximize predicted specificity.