Housheng Hansen He

Abstract Cohesin organizes three-dimensional genome architecture by extruding DNA loops, but the mechanisms specifying its chromatin entry sites remain unclear. Here we show that the cohesin loader NIPBL is prepositioned at highly tissue-specific sites, largely distinct from the broadly conserved binding profiles of CTCF and cohesin.

Abstract Therapies for chronic and end-organ diseases are often associated with systemic toxicity, and such conditions result in difficulty treating patients. RNA nanomedicine has demonstrated unprecedented advantages in the field of therapeutics through its selective organ targeting, personalized treatment option and safety.

Abstract Cell-free DNA analysis via methylation and fragmentation profiling has advanced minimally invasive cancer detection; however, broader application has been limited by small cohorts and inconsistent data processing. Here we collated 1,074 cfMeDIP-seq profiles across 9 studies, comprising cancer samples from 11 cancer types, carriers of Li-Fraumeni syndrome and healthy controls. We developed a uniform computational workflow to mitigate technical and biological confounders across cohorts.