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Articles by Markus Sauer
Sphingolipids associate with the chlamydial nucleoid and mark developmental transitions in Chlamydia trachomatis
Abstract Chlamydia trachomatis is an obligate intracellular bacterial pathogen and a leading cause of sexually transmitted infections worldwide. During its biphasic developmental cycle, infectious, non-replicative elementary bodies alternate with replicative reticulate bodies within a membrane-bound intracellular niche known as the inclusion. C.
Resolving endogenous protein organization in cells with nanometer resolution
Abstract Latest refinements in super-resolution microscopy achieved spatial resolutions in the one nanometer range. However, improvement of localization precision advanced faster than labeling methods impeding the translation of such high resolutions to cells. Hence, imaging of the nano-architecture of endogenous multiprotein complexes remains challenging.
RBM20 variants disrupt Ca 2+ handling and metabolism in dilated and non-compaction cardiomyopathy stem cell models
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Abstract Mutations in the splice-regulator RBM20 cause heart failure with reduced ejection fraction (HFrEF), typically manifesting as dilated cardiomyopathy (DCM). Mutations at position 634 in the RS-domain cause DCM with (R634L) or without (R634W) left ventricular non-compaction (LVNC). However, the mechanisms underlying phenotype variability and personalized therapy beyond HFrEF remain unclear.
By Sabine Rebs, Farbod Sedaghat-Hamedani, Elham Kayvanpour, Hanna Eberl, Jan Dudek, Julia Unsöld, Christoph Reich, Teresa Klein, Sören Doose, Viacheslav O. Nikolaev, Ahmed Wagdi, Michael Kohlhaas, Nataliya Dybkova, Patrícia Costa, Kaomei Guan, Gerd Hasenfuss, Eva A. Rog-Zielinska, Samuel Sossalla, Markus Sauer, Christoph Maack, Malte Tiburcy, Benjamin Meder, Nicole Wagensohner, Aylin Seedorf, Daniela Hübscher, Tjark Buchwald
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Nature
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Energy transfer leaves fingerprints in cyanine photoswitching behavior
Loading metrics Open Access Peer-reviewed Research Article ? This is an uncorrected proof. Citation: Ebert V, Sauer M, Doose S (2026) Energy transfer leaves fingerprints in cyanine photoswitching behavior. PLoS Comput Biol 22(5): e1014322. https://doi.org/10.1371/journal.pcbi.1014322 Editor: Joshua N. Milstein, University of Toronto Mississauga, CANADA Received: March 16, 2026; Accepted: May 11, 2026; Published: May 18, 2026 Copyright: © 2026 Ebert et al.
DNAM-1 mediates NK-cell activation and host-pathogen interaction via direct binding to fungal cell wall proteases
Abstract Natural killer (NK) cells play a key role in combating invasive mycoses, with surface proteins crucial for immune cell-target interactions. Here, we investigate the role of DNAX accessory molecule 1 (DNAM-1; CD226) in the recognition of Aspergillus fumigatus and Candida albicans hyphae. Super-resolution microscopy reveals a uniform distribution of DNAM-1 on naïve NK cells that is maintained upon contact with fungal hyphae.
Loss of GPRC5D enhances the proliferative capacity and competitive fitness of myeloma upon anti-GPRC5D immunotherapy
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Abstract Immunotherapies targeting surface antigens have transformed the treatment landscape of multiple myeloma (MM), with GPRC5D emerging as a promising therapeutic target. Monoallelic loss of GPRC5D is frequently observed in newly diagnosed MM patients, and the incidence of acquired GPRC5D alterations increases following exposure to GPRC5D-directed therapies.
By Umair Munawar, Thomas Nerreter, Alexander M. Leipold, Seungbin Han, Christina Verbruggen, Elena Gerhard-Hartmann, Cornelia Vogt, Sabine Roth, Julia Weingart, Patrick Eiring, Marietta Truger, Nazia Afrin, Torsten Steinbrunn, Xiang Zhou, Johanna Lehmann, Max Koppel, Andreas Rosenwald, Claudia Haferlach, Michael Hudecek, Antoine-Emmanuel Saliba, Leo Rasche, Markus Sauer, Hermann Einsele, Bernhard Kuster, Johannes M. Waldschmidt, K. Martin Kortüm, Johanna Thurner, Silvia Nerreter, Björn Grams, Shilpa Kurian, Emma Besant, Yoko Tamamushi, Nina Rein
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Nature
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Adventitious carbon breaks symmetry in oxide contact electrification
Abstract Insulating oxides are among the most abundant solid materials in the universe1,2,3. Of the many ways in which they influence natural phenomena, perhaps the most consequential is their capacity to transfer electrical charge during contact4,5,6,7,8,9,10—which occurs even between samples of the same oxide—yet the symmetry-breaking parameter that causes this remains unidentified11,12.
Single-molecule localization microscopy reveals the molecular organization of endogenous membrane receptors
Abstract Super-resolution microscopy in combination with genetic labeling methods allows imaging of single proteins in cells. However, visualizing endogenous proteins on primary cells remains challenging due to the use of sterically demanding antibodies for labeling. Here, we demonstrate how immunolabeling conditions and antibody cross-linking influence the quantification and identification of membrane receptor stoichiometry on cells using single-molecule localization microscopy.
PSMA PET Evaluation with a Deep Learning Platform Compared with a Standard Image Viewer and Histopathology
Standardized prostate-specific membrane antigen (PSMA) PET/CT evaluation and reporting was introduced to aid interpretation, reproducibility, and communication. Artificial intelligence may enhance these efforts. This study aimed to evaluate the performance of aPROMISE, a deep learning segmentation and reporting software for PSMA PET/CT, compared with a standard image viewer (IntelliSpace Portal [ISP]) in patients undergoing PSMA-radioguided surgery.
Improving single molecule localisation microscopy reconstruction by extending the temporal context
Abstract Single-molecule localization microscopy methods such as dSTORM require specific buffer conditions to enable blinking and detection of individual emitters, making them incompatible with live cell imaging and expansion microscopy. An alternative approach to achieve super-resolution without blinking is to observe the fluctuations of the emitter intensity over time.
Super-resolution microscopy - photophysical implications and applications
Nyckelord: NATURVETENSKAP; NATURAL SCIENCES; Biological and Biomedical Physics; Biologisk och biomedicinsk fysik; Sammanfattning: Unparalleled specificity and high sensitivity have made fluorescence microscopy an indispensable tool for life sciences. Both these aspects come from the use of light-emitting fluorophores, labeled to molecules and structures of interest.
One-step nanoscale expansion microscopy reveals individual protein shapes - Nature Biotechnology
Abstract The attainable resolution of fluorescence microscopy has reached the subnanometer range, but this technique still fails to image the morphology of single proteins or small molecular complexes. Here, we expand the specimens at least tenfold, label them with conventional fluorophores and image them with conventional light microscopes, acquiring videos in which we analyze fluorescence fluctuations.
Trifunctional sphingomyelin derivatives enable nanoscale resolution of sphingomyelin turnover in physiological and infection processes via expansion microscopy | Nature Communications
Abstract Sphingomyelin is a key molecule of sphingolipid metabolism, and its enzymatic breakdown is associated with various infectious diseases. Here, we introduce trifunctional sphingomyelin derivatives that enable the visualization of sphingomyelin distribution and sphingomyelinase activity in infection processes.
Decoding the molecular interplay of endogenous CD20 and Rituximab with fast volumetric nanoscopy
Abstract Elucidating the interaction between membrane proteins and antibodies requires fast whole-cell imaging at high spatiotemporal resolution. Lattice light-sheet (LLS) microscopy offers fast volumetric imaging but suffers from limited spatial resolution. DNA-PAINT achieves molecular resolution but is practically restricted to two-dimensional imaging due to long acquisition times.
Impaired FADD/BID signaling mediates cross-resistance to immunotherapy in Multiple Myeloma - Communications Biology
Abstract The treatment landscape in multiple myeloma (MM) is shifting from genotoxic drugs to immunotherapies. Monoclonal antibodies, immunoconjugates, T-cell engaging antibodies and CART cells have been incorporated into routine treatment algorithms, resulting in improved response rates. Nevertheless, patients continue to relapse and the underlying mechanisms of resistance remain poorly understood.
Fast, whole-cell DNA-PAINT imaging with dual-labeled self-quenched imager probes
Abstract In DNA-PAINT, background fluorescence emerging from unbound imager probes requires the use of low imager concentrations, reducing the imaging speed substantially. Here, we present a novel class of dual-labeled probes that show strong fluorescence quenching when unbound, enabling imaging at higher probe concentrations with an up to ~15-fold increase in imaging speed and improved resolution. We demonstrate two-dye imager DNA-PAINT (TDI-DNA-PAINT) imaging in cells and on DNA origami.
Tumor-derived GDF-15 blocks LFA-1 dependent T cell recruitment and suppresses responses to anti-PD-1 treatment - Nature Communications
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Proteins and antibodies HIS-tagged recombinant human Growth and Differentiation Factor-15 was produced in HEK293T cells (Invigate GmbH, Jena). The anti-human GDF-15 antibody used in this manuscript was generated in female C57BL/6J GDF-15-/–mice kindly provided by Dr. Jens Strelau (Heidelberg, Germany) and validated for binding affinity by surface plasmon resonance at NMI (Reutlingen, Germany). Specificity was tested by tissue cross-reactivity studies.
By Carmen Reitinger, Kilian Wistuba-Hamprecht, Hubert Hackl, Hemant R. Joshi, Phil Cheng, Mitchell Levesque, Matthias Goebeler, Markus Sauer, Falk Nimmerjahn, Stefanie von Felten, Michel Mittelbronn, Andreas Beilhack, Reinhard Dummer, Markus Haake, Lena Sorger, Jacob Späth, Rudi Beschorner, Alexander Thiem, Manfred Rüdiger
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Nature
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Trifunctional linkers enable improved visualization of actin by expansion microscopy
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Schärfer als das Mikroskop erlaubt
Zusammenfassung Mit Hilfe der hochauflösenden Fluoreszenzmikroskopie lassen sich Bilder mit einer räumlichen Auflösung von rund 20 nm, weit unterhalb der Beugungsgrenze, aufnehmen. Eine weitere Verbesserung der räumlichen Auflösung bis in den molekularen Bereich ist jedoch durch die Interaktion der Farbstoffe miteinander erschwert.
Feasibility of 99mTc-MIP-1404 for SPECT/CT Imaging and Subsequent PSMA-Radioguided Surgery in Early Biochemically Recurrent Prostate Cancer: A Case Series of 9 Patients
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Filamin A organizes γ‑aminobutyric acid type B receptors at the plasma membrane - Nature Communications
Abstract The γ-aminobutyric acid type B (GABAB) receptor is a prototypical family C G protein-coupled receptor (GPCR) that plays a key role in the regulation of synaptic transmission. Although growing evidence suggests that GPCR signaling in neurons might be highly organized in time and space, limited information is available about the mechanisms controlling the nanoscale organization of GABAB receptors and other GPCRs on the neuronal plasma membrane.
MYC multimers shield stalled replication forks from RNA polymerase - Nature
Abstract Oncoproteins of the MYC family drive the development of numerous human tumours1. In unperturbed cells, MYC proteins bind to nearly all active promoters and control transcription by RNA polymerase II2,3. MYC proteins can also coordinate transcription with DNA replication4,5 and promote the repair of transcription-associated DNA damage6, but how they exert these mechanistically diverse functions is unknown.
ShareLoc — an open platform for sharing localization microscopy data - Nature Methods
Author notes Samuel F. H. Barnett Present address: Max Planck Institute for Medical Research, Heidelberg, Germany These authors contributed equally: Wei Ouyang, Jiachuan Bai.
Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier - Nature Methods
Abstract Advances in super-resolution microscopy have demonstrated single-molecule localization precisions of a few nanometers. However, translation of such high localization precisions into sub-10-nm spatial resolution in biological samples remains challenging. Here we show that resonance energy transfer between fluorophores separated by less than 10 nm results in accelerated fluorescence blinking and consequently lower localization probabilities impeding sub-10-nm fluorescence imaging.
ReCSAI: Recursive compressed sensing artificial intelligence for confocal lifetime localization microscopy
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ReCSAI Recursive compressed sensing artificial intelligence for confocal lifetime localization microscopy
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Selective inhibition of miRNA processing by a herpesvirus-encoded miRNA - Nature
Abstract Herpesviruses have mastered host cell modulation and immune evasion to augment productive infection, life-long latency and reactivation1,2. A long appreciated, yet undefined relationship exists between the lytic–latent switch and viral non-coding RNAs3,4.
[Ru(tmphen)3]2[Fe(CN)6] and [Ru(phen)3][Fe(CN)5(NO)] complexes; Heterostructured RuO2-Fe2O3 nanocomposite: an efficient alkaline HER and OER electrocatalyst - Dalton Transactions (RSC Publishing)
[Ru(tmphen)3]2[Fe(CN)6] and [Ru(phen)3][Fe(CN)5(NO)] complexes; Heterostructured RuO2–Fe2O3 nanocomposite: an efficient alkaline HER and OER electrocatalyst Hamta Mosallaei, Hassan Hadadzadeh, Annette Foelske, Markus Sauer, Hadi Amiri Rudbari and Olivier Blacque Abstract Water electrolysis is one the most capable processes to supply clean fuel.
Photoblueing of organic dyes can cause artifacts in super-resolution microscopy
Abstract Illumination of fluorophores can induce a loss of the ability to fluoresce, known as photobleaching. Interestingly, some fluorophores photoconvert to a blue-shifted fluorescent molecule as an intermediate on the photobleaching pathway, which can complicate multicolor fluorescence imaging, especially under the intense laser irradiation used in super-resolution fluorescence imaging.
Superagonistic CD28 stimulation induces IFN‐γ release from mouse T helper 1 cells in vitro and in vivo
Letter to the Editor Niklas Beyersdorf Corresponding Author niklas.beyersdorf@vim.uni-wuerzburg.de orcid.org/0000-0002-9236-5415 Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany PD Dr. med. Niklas Beyersdorf, Institute for Virology and Immunobiology, Versbacher Str.
DHX36 prevents the accumulation of translationally inactive mRNAs with G4-structures in untranslated regions
Abstract Translation efficiency can be affected by mRNA stability and secondary structures, including G-quadruplex structures (G4s). The highly conserved DEAH-box helicase DHX36/RHAU resolves G4s on DNA and RNA in vitro, however a systems-wide analysis of DHX36 targets and function is lacking.
Registration and visualization of correlative super-resolution microscopy data
Sebastian Reinhard † Author Footnotes † The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors. Sebastian Reinhard Footnotes † The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.
Carbon-based SILP catalysis for the selective hydrogenation of aldehydes using a well-defined Fe(II) PNP complex
In this work, the supported ionic liquid phase (SILP) method was applied for the immobilization of a newly developed, well-defined hydride Fe(II) PNP pincer complex dissolved an in ionic liquid (IL) onto polymer-based spherical activated carbon. This novel SILP catalyst was structurally characterized by electron microscopy, N2 adsorption–desorption, FTIR, and XPS measurements and was used for the hydrogenation of aldehydes to alcohols.
Single-molecule localization microscopy comes of age: From concepts to biological impact
Superresolution microscopy by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cells function at a molecular level. Single-molecule localization microscopy (SMLM) can provide single-molecule information about the distribution and even the absolute numbers of proteins in subcellular compartments.
Super-resolving Microscopy in Neuroscience
SPECIAL ISSUE This article is part of the Frontiers of Analytical Science special issue. 1. Introduction Imaging technologies are central platforms that drive fundamental research in virtually all disciplines across the life sciences. In neuroscience, confocal microscopy constituted the standard fluorescence imaging technology for analysis of neuronal tissue during the last decades.
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