Matthew Bochman

KEYWORDS DNA binding protein DNA-protein interaction molecular biology Saccharomyces cerevisiae telomere Cdc13 dynamic DNA exchange OB fold ABBREVIATIONS ssDNA (single-stranded DNA) ssBP (ssDNA binding protein) CST (Cdc13-Stn1-Ten1 complex) DDE (dynamic DNA exchange) BLI (biolayer interferometry) IR700 and IR800 (fluorophores with emission wavelengths near 700 and 800 nm) TelXXG (telomeric repeat sequence G-strand of XX nucleotides in length) INTRODUCTION Telomeres protect chromosome ends...

Abstract Single-stranded DNA (ssDNA) binding proteins (ssBPs) are essential in eukaryotes to protect telomeres from nuclease activity. In Saccharomyces cerevisiae, the ssBP Cdc13 is an essential protein that acts as a central regulator of telomere length homeostasis and chromosome end protection, both alone and as part of the Cdc13-Stn1-Ten1 (CST) complex. Cdc13 has high binding affinity for telomeric ssDNA, with a very slow off-rate.

Onyekachi E. Ononye1‡, Christopher W. Sausen2‡, Lata Balakrishnan1* and Matthew L. Bochman2* 1Department of Biology, School of Science, Indiana University, Purdue University Indianapolis, Indianapolis, Indiana, USA 2Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, Indiana, USA ↵*For correspondence: Lata Balakrishnan, latabala{at}iupui.edu; Matthew L. Bochman, bochman{at}indiana.edu. ↵‡ These authors contributed equally to this work.