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Multiscale Biology

As seen in: Nature, Oxford Academic, Frontiers, heart, Genome Biology, Genome Medicine, JAMA Psychiatry, Science Magazine, Cell Press, Cell Reports and
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Articles

Inhibition of endothelial to mesenchymal transition in a large animal preclinical arterio-venous fistula model leads to improved remodeling and reduced stenosis

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By Johan L. M, Karolinska Universitetssjukhuset, Multiscale Biology
| Oxford Academic Verified
Vein grafts are used for many indications, including bypass graft surgery and arterio-venous fistula (AVF) formation. However, patency following vein grafting or AVF formation is suboptimal for various reasons, including thrombosis, neointimal hyperplasia and adverse remodeling. Recently, endothelial to mesenchymal transition (EndMT) was found to contribute to neointimal hyperplasia in mouse vein grafts.
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Maintenance of cellular vitamin B6 levels and mitochondrial oxidative function depend on pyridoxal 5’-phosphate homeostasis protein (PLPHP)

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By Johan Gerrits, Multiscale Biology, Hans R. Waterham, Ronald Wanders
| The Journal of Biological Chemistry Verified
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DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing - Genome Biology

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By Multiscale Biology
| Genome Biology Verified
Plasmids and yeast strains Plasmids The hADAR2 coding sequences were amplified from cDNA of 293 T cells with primer hADAR2-P5 and hADAR2-P3 (Additional file 2: Table S3). The plasmid pDUAL-HFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan). PCR was performed using KOD FX DNA polymerase (TOYOBO).
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