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ArticleOnline nowOpen access Affiliations & Notes 1Institute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, 94158, United States of America 2UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, CA, 94158, United States of America 3Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, United States of America 4Department of Electrical Engineering and...
Abstract Studies of genome regulation routinely use high-throughput DNA sequencing approaches to determine where specific proteins interact with DNA, and they rely on DNA amplification and short-read sequencing, limiting their quantitative application in complex genomic regions. To address these limitations, we developed directed methylation with long-read sequencing (DiMeLo-seq), which uses antibody-tethered enzymes to methylate DNA near a target protein’s binding sites in situ.
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