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Abstract Primary human myeloid cells hold promise for immunotherapies, yet efficient, scalable technologies for engineering and screening in these cells remain limited. Here we present a virus-like particle (VLP)-based toolkit that delivers diverse CRISPR editing modalities to human monocytes, macrophages and dendritic cells with high efficiency while preserving viability and innate immune responsiveness.
ArticleOnline nowOpen access Affiliations & Notes 1Institute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, 94158, United States of America 2UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, CA, 94158, United States of America 3Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, United States of America 4Department of Electrical Engineering and...
Abstract Understanding biological systems requires observing features and processes across vast spatial and temporal scales, spanning nanometers to centimeters and milliseconds to days, often using multiple imaging modalities within complex native microenvironments.
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