Objective: To assess the safety and efficacy of lyophilized, milled, human amnion on wound healing in an equine distal limb wound model in a blinded controlled study. Animals: Four clinically normal adult horses (3 Thoroughbred and 1 Paint, age 6-19 years) obtained via donation. Institutional Animal Care and Use Committee approval was obtained for this study. Procedures: One forelimb of each horse was randomly assigned to the treatment group, and the contralateral limb was assigned as the control.