Seon S. Kinrot

Abstract T cell receptor (TCR) gene therapy is a potent form of cellular immunotherapy in which patient T cells are genetically engineered to express TCRs with defined tumor reactivity. However, the isolation of therapeutic TCRs is complicated by both the general scarcity of tumor-specific T cells among patient T cell repertoires and the patient-specific nature of T cell epitopes expressed on tumors.

Highlights • Massively multiplexed FISH enables mapping chromatin structure at genome scale • Multimodal high-throughput imaging places chromatin structure in functional context • Trans-chromosome or long-range interactions occur preferentially among active chromatin • Transcription activity correlates with local enrichment of compartment A chromatin Summary The 3D organization of chromatin regulates many genome functions.

Highlights • Massively multiplexed FISH enables mapping chromatin structure at genome scale • Multimodal high-throughput imaging places chromatin structure in functional context • Trans-chromosome or long-range interactions occur preferentially among active chromatin • Transcription activity correlates with local enrichment of compartment A chromatin Summary The 3D organization of chromatin regulates many genome functions.