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Abstract Engineering functional tissues for transplantation requires insight into epigenetic mechanisms that regulate stem cell fate. We have developed the Wnt-induced osteogenic tissue model (WIOTM), a platform that recapitulates human osteogenesis, and identified acetylation of histone H3 at lysine 14 (H3K14ac) as a critical epigenetic regulator in human skeletal stem cells (hSSCs).
ArticleOnline nowOpen access 1Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK 2Division of Biology and Biological Engineering, California Institute of Technology (Caltech), Pasadena, CA 91125, USA 3Department of Bioengineering and Barnett Institute, Northeastern University, Boston, MA 02115, USA 4Parallel Squared Technology Institute, Watertown, MA 02472, USA 5Department of Biochemistry, University of Cambridge, Hopkins...
Keywords blastoid blastocyst screening xenobiotics trophectoderm epiblast primitive endoderm GATA4 embryo models Introduction Integrated embryo-like models generated by assembling embryonic and extraembryonic stem cells1,2 offer a more accessible and experimentally tractable alternative to natural mammalian embryos for mechanistic studies.
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