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Shivanshi Vaid on Muck Rack

Shivanshi Vaid

As seen in: Stem Cell Reports
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Articles

Delayed forebrain excitatory and inhibitory neurogenesis in STRADA-related megalencephaly via mTOR hyperactivity

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By Tong Pan, Grace A. Lin, Xuan Li, John Walker, Amrita Kondur, Julie Sturza, Ken Inoki, Tomozumi Imamichi, Weizhong Chang, Louis T. Dang, Debora VanHeyningen, Sahej Kohli, Aiswarya Saravanan, Daniel C. Jaklic, Saul Pantoja-Gutierrez, Shivanshi Vaid
| Stem Cell Reports Verified
Results To avoid confounding from chromosomal abnormalities in prior PMSE patient iPSC lines (Dang et al., 2021), we used CRISPR/Cas9 to generate STRADA loss-of-function KO lines with compound heterozygous truncating variants and isogenic controls (Figures S1A and S1B). Lines expressed pluripotency markers (Figure S1C) and showed no karyotypic abnormalities (data not shown). Two STRADA KO (M1 and M2) and two isogenic control (C1 and C2) lines were used for all experiments.
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