Sreekala Nampoothiri

Immunohistology For human hypothalamus immunolabeling, a citrate-buffer antigen retrieval step, 10mM Citrate in TBS-Triton 0.1% pH 6 for 30 min at 70°C, was performed on 20μm sections. After 3 washes of 5 min with TBS-Triton 0.1%, sections were blocked in incubation solution (ICS: 10% normal donkey serum, 3 mg/mL BSA in TBS-Triton 0.3% pH 7,4) for 1 h. Blocking was followed with primary antibody incubation (Table S3) in ICS for 48h at 4°C.

Abstract The hypothalamic arcuate nucleus (ARH) contains neurons vital for maintaining energy homeostasis that sense and respond to changes in blood-borne metabolic hormones. Despite its juxtaposition to the median eminence (ME), a circumventricular organ lacking a blood-brain barrier and thus exposed to circulating molecules, only a few ventral ARH neurons perceive these extravasating metabolic signals due to a poorly understood ME/ARH diffusion barrier.

Abstract The human nasal region arises from neural crest and placodal lineages, yet its early development remains poorly understood owing to limited fetal tissue access and structural complexity. Here we present an integrated single-nucleus and spatial transcriptomic atlas of the human fetal nasal region, generated from male and female fetuses between 7 and 12 post-conceptional weeks.