Pascal Barbry

Abstract Sex-specific differences in metabolic and immune function are shaped by endocrine–immune interactions, yet the complexity of this relationship remains poorly understood. Here, we identify an immune cell-like state of tanycytes, an ependymoglial cell type lining the third ventricle that mediates brain–body communication1,2 in both mice and humans.

Immunohistology For human hypothalamus immunolabeling, a citrate-buffer antigen retrieval step, 10mM Citrate in TBS-Triton 0.1% pH 6 for 30 min at 70°C, was performed on 20μm sections. After 3 washes of 5 min with TBS-Triton 0.1%, sections were blocked in incubation solution (ICS: 10% normal donkey serum, 3 mg/mL BSA in TBS-Triton 0.3% pH 7,4) for 1 h. Blocking was followed with primary antibody incubation (Table S3) in ICS for 48h at 4°C.

Abstract Aging increases the risk of developing fibrotic diseases by hampering tissue regeneration after injury. Using longitudinal single-cell RNA-seq and spatial transcriptomics, here we compare the transcriptome of bleomycin (BLM) -induced fibrotic lungs of young and aged male mice, at 3 time points corresponding to the peak of fibrosis, regeneration, and resolution. We find that lung injury shifts the transcriptomic profiles of three pulmonary capillary endothelial cells (PCEC) subpopulations.