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Tanycytic degeneration impairs tau clearance and contributes to Alzheimer’s disease pathology
Immunohistology For human hypothalamus immunolabeling, a citrate-buffer antigen retrieval step, 10mM Citrate in TBS-Triton 0.1% pH 6 for 30 min at 70°C, was performed on 20μm sections. After 3 washes of 5 min with TBS-Triton 0.1%, sections were blocked in incubation solution (ICS: 10% normal donkey serum, 3 mg/mL BSA in TBS-Triton 0.3% pH 7,4) for 1 h. Blocking was followed with primary antibody incubation (Table S3) in ICS for 48h at 4°C.
Aging affects reprogramming of pulmonary capillary endothelial cells after lung injury in male mice - Nature Communications
Abstract Aging increases the risk of developing fibrotic diseases by hampering tissue regeneration after injury. Using longitudinal single-cell RNA-seq and spatial transcriptomics, here we compare the transcriptome of bleomycin (BLM) -induced fibrotic lungs of young and aged male mice, at 3 time points corresponding to the peak of fibrosis, regeneration, and resolution. We find that lung injury shifts the transcriptomic profiles of three pulmonary capillary endothelial cells (PCEC) subpopulations.
Épidémiologie moléculaire par séquençage des acides nucléiques d’origine virale présents dans les eaux usées
Accueil Tous les numéros Volume 41 / Numéro 6-7 (Juin-Juillet 2025) Med Sci (Paris), 41 6-7 (2025) 585-592 Full HTML Numéro Med Sci (Paris) Volume 41, Numéro 6-7, Juin-Juillet 2025 Page(s) 585 - 592 Section Repères DOI https://doi.org/10.1051/medsci/2025083 Publié en ligne 7 juillet 2025 Med Sci (Paris) 2025; 41 : 585–592 Épidémiologie moléculaire par séquençage des acides nucléiques d’origine virale présents dans les eaux usées 1,2, 1,2, 1,3, 1,3, 1,3 et 1,3,4* 1 Université Côte d’Azur, CNRS...
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