Weng Hua Khoo

Abstract Musculoskeletal diseases are a major health burden. Development of bone-active therapies has been hindered by limited understanding of the cells and genes that regulate the skeleton. We exploited the value of cross-species analysis and developed single-cell methodologies in skeletal tissues to define the critical endosteal compartment that regulates bone turnover.

Keywords memory vaccine germinal center plasma cells broadly neutralizing antibodies affinity maturation immune imprinting original antigenic sin SARS-CoV-2 subcapsular sinus macrophage Introduction Vaccines protect from recurrent infection and disease by inducing the secretion of neutralizing antibodies by long-lived plasma cells (PCs)1 and by generating memory B cells (Bmems).2 Long-lived PCs are terminally differentiated and can only secrete antibodies of fixed specificity and affinity for...

Abstract Intravital two-photon microscopy enables deep-tissue imaging at high temporospatial resolution in live animals. However, the endosteal bone compartment and underlying bone marrow pose unique challenges to optical imaging as light is absorbed, scattered and dispersed by thick mineralized bone matrix and the adipose-rich bone marrow. Early bone intravital imaging methods exploited gaps in the cranial sutures to bypass the need to penetrate through cortical bone.