Yining Hao
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Mining of natural diversity enables efficient and expressible peptide asparaginyl ligases
Abstract Peptide asparaginyl ligases (PALs) are powerful tools for protein engineering but are limited by natural rarity and poor expression. We mine 23 cyclotide-rich Viola species, uncovering 29 PALs that expand the known repertoire to 47. A dual-objective screen identifies VdiPAL1 as the best-performed natural PAL, with twice efficiency of wt-VyPAL2 and 12 mg L-1 soluble expression in E. coli.
Fatigue Behavior and Multiscale Structural Evolution of Polyamide 66 Industrial Fiber Under Cyclic Loading
Conflicts of Interest The authors declare no conflicts of interest. Data Availability Statement Data will be made available on request. Supporting Information Filename Description pen70348-sup-0001-Supinfo.docxWord 2007 document , 2.7 MB Data S1: pen70348-sup-0001-Supinfo.docx. References 1, , , et al., “Thermal Aging of a Polyamide 66—Experimental Analysis and Modeling,” Journal of the Brazilian Society of Mechanical Sciences and Engineering 43, no. 2 (2021): 92–102.
Bioaccessibility of Phyllanthus emblica L. extract and its potential as a functional food for protecting against inflammatory bowel disease
CONFLICT OF INTEREST The authors declare no conflicts of interest. DATA AVAILABILITY STATEMENT The data that support the findings of this study are available from the corresponding author upon reasonable request. Supporting Information Filename Description jsfa70399-sup-0001-Supinfo.docxWord 2007 document , 15.2 KB Data S1 REFERENCES 1, , , , , et al., Phyllanthus emblica Linn: a comprehensive review of botany, traditional uses, phytonutrients, health benefits, quality markers, and applications.
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