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Ariana Barcoma

As seen in: Cell Reports, Cell Press
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Articles

Single-cell epigenomics uncovers heterochromatin instability and transcription factor dysfunction during mouse brain aging

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By Maria Amaral, Sainath Mamde, MICHAEL Miller, Xiaomeng Hou, Jessica Arzavala, Julia Osteen, Nicholas Johnson, Qian Yang, Emily Eisner, Qiurui Zeng, Cindy Tatiana Báez-Becerra, Joseph Kern, Jonathan A. Rink, Ariana Barcoma, Silvia Cho, Stella Cao, Nora E. Emerson, Jasper Lee, Jackson Willier, Songpeng Zu, Quan Zhu, Sebastian Preissl, Allen Wang, Joseph R. Ecker, Bing Ren, Elizabeth Walker Smoot, Jacqueline Olness, Timothy Loe, Henry Jiao, Maria Margarita Behrens
| Cell Reports Verified
STAR★Methods REAGENT or RESOURCESOURCEIDENTIFIER Chemicals, peptides, and recombinant proteins Tn5 transposase QB3 Macrolab at UC Berkeley N/A SPRISelect reagent Beckman Coulter Cat# B23319 DRAQ7 Cell Signaling Cat# 7406 NEBNext High-Fidelity PCR Master Mix NEB Cat# M0541L LightCycler 480 SYBR Green I Master Mix Roche Cat# 04707516001 DTT nuclei permeabilization buffer Sigma-Aldrich Cat# 646563-10X IGEPAL-CA630 Sigma-Aldrich Cat#I8896 1X cOmplete EDTA-free protease inhibitor cocktail tablets...
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Cell-type-specific transposon demethylation and TAD remodeling in aging mouse brain

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By Qiurui Zeng, Wenliang Wang, Wei Tian, Amit Klein, Anna Bartlett Verified, Hanqing Liu, Joseph R. Nery, Rosa Castanon, Julia Osteen, Nicholas Johnson, Wubin Ding, Huaming Chen, Jordan Altshul, Mia Kenworthy, Cynthia Valadon, William Owens, Zhanghao Wu, Maria Amaral, Nathan R. Zemke, Yuru Song, Silvia Cho, Jonathan A. Rink, Jasper Lee, Nora E. Emerson, Yuancheng Lu, Bing Ren, M. Margarita Behrens, Joseph R. Ecker, Cindy Tatiana Báez-Becerra, Chumo Chen, Jackson Willier, Stella Cao, Ariana Barcoma, Jessica Arzavala
| Cell Press Verified
Results We refined and employed snmC-seq3,35,36 an advanced single-nucleus methylome sequencing technique, to achieve base-resolution profiling of genome-wide 5-methylcytosine (5mC) across eight brain regions in C57BL/6 male mice (Figure 1A).
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