Chaoying Liang

Isolation of LP and mLN immune cells Immune cells were isolated from the small intestinal lamina propria and mesenteric lymph nodes as previously described.31 For intestinal preparations, Peyer’s patches were excised, and the tissue was flushed with 10 mL ice-cold PBS, opened longitudinally, cut into ∼2 cm pieces, and washed three times by vortexing in 10 mL PBS with 5% FBS for 2 minutes each, using a multitube vortexer.

Abstract T cells have important functions in development and disease processes through T cell receptor (TCR)-dependent activities. Many tools were developed to predict the binding between TCRs and antigens. However, one of the uncertainties is whether such tools can decipher how small changes in the TCRs or antigenic peptides contribute to binding. We develop a deep learning model, pMTnet-omni, which not only predicts the binding vs.