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Abstract In principle, induced pluripotent stem cells (iPSCs) can differentiate into any cell type in the body. The challenge is to find a way to rapidly expand the dimensionality of cell types and cell states we can characterize. To address this, we developed a guided differentiation protocol to produce heterogeneous differentiating cultures of cardiac cell types (cardiac HDCs) in 16 days.
ResourceVolume 19, Issue 8p1217-1232Open access 1Centre for Stem Cell Biology, School of Biosciences, The University of Sheffield, Sheffield, UK 2Neuroscience Institute, The University of Sheffield, Sheffield, UK 3INSIGNEO Institute, The University of Sheffield, Sheffield, UK 4WiCell Research Institute, Madison, WI, USA 5Department of Human Genetics, University of Chicago, Chicago, IL, USA 6Department of Human Molecular Genetics and Biochemistry, Faculty of Medicine, Tel Aviv University, Tel...
Abstract We have developed a guided differentiation protocol for induced pluripotent stem cells (iPSCs) that rapidly generates a temporally and functionally diverse set of cardiac-relevant cell types. By leveraging techniques used in embryoid body and cardiac organoid generation, we produce both progenitor and terminal cardiac cell types concomitantly in just 10 days.
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