Ivana Barbaric

Main text In 2023, the International Society for Stem Cell Research (ISSCR) published its document “Standards for Human Stem Cell Use in Research” (https://www.isscr.org/basic-research-standards), a guide to best practice in laboratory use of human pluripotent stem cells (PSCs) and tissue stem cells (TSCs). The document was the result of nearly 2 years of work by an international committee of experts, ISSCR staff, and outside reviewers (Lefkopoulos, 2023; Ludwig et al., 2023).

ResourceVolume 19, Issue 8p1217-1232Open access 1Centre for Stem Cell Biology, School of Biosciences, The University of Sheffield, Sheffield, UK 2Neuroscience Institute, The University of Sheffield, Sheffield, UK 3INSIGNEO Institute, The University of Sheffield, Sheffield, UK 4WiCell Research Institute, Madison, WI, USA 5Department of Human Genetics, University of Chicago, Chicago, IL, USA 6Department of Human Molecular Genetics and Biochemistry, Faculty of Medicine, Tel Aviv University, Tel...

INTRODUCTION Regenerative therapies involving the transplantation of hPSC-derived tissues require the propagation of billions of cells, necessitating prolonged in vitro culture to meet this demand.[1] However, observationally, hPSCs that are grown over high passage numbers tend to acquire genetic changes that bestow them with altered phenotypes.[2] In turn, if such genetic changes are not routinely detected and eliminated during the production of cells for therapies or bioengineered tissues,...