Gabriel D. Victora

GCs select consistently for increases in affinity and maintenance of Ig expression Whereas GC phylogenies varied widely in structure (Figures 1C, 1D, and S2), replay GCs were much more consistent with respect to affinity maturation. Median affinity was higher than naive in 117 of 119 GCs, whereas variance was relatively low (0.87 ± 0.38 at 15 dpi and 1.00 ± 0.34 at 20 dpi [mean ± SD]; Figures 4A and S5A).

Keywords B cell germinal center antibody-mediated feedback influenza hemagglutinin plasma cell Introduction Affinity maturation of antibody responses takes place in germinal centers (GCs), microanatomical structures located within secondary lymphoid organs, where B cells undergo somatic hypermutation of their B cell receptors (BCRs) followed by affinity-dependent clonal expansion and differentiation into memory B cells and plasma cells (PCs).1,2,3,4 Entry of B cells into GCs and their...

ArticleOnline nowOpen access 1Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA 2Institute for Immunology and Immune Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA 3Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA 4Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy 5Department of Pathology, University of Massachusetts Chan...