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Keywords CRISPR cancer immunology tumor-associated macrophage Zeb2 deep generative modeling single cell genomics regulatory network TAM reprogramming cancer immunotherapy systems immunolgy Get full text access Log in, subscribe or purchase for full access. References 1. Katkar, G. ∙ Ghosh, P. Macrophage states: there’s a method in the madness Trends Immunol. 2023; 44:954-964 2. Wynn, T.A. ∙ Chawla, A. ∙ Pollard, J.W. Macrophage biology in development, homeostasis and disease Nature. 2013; 496:445-455 3.
ArticleVolume 187, Issue 1p149-165.e23Open Archive 1Department of Systems Immunology, Weizmann Institute of Science, 7610001 Rehovot, Israel 2Department of Neurology, Clinical Neuroscience Center, University Hospital Zurich and University of Zurich, Zurich, Switzerland 3Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA 4Department of Stem Cell Transplantation and Cellular Therapy, the University of Texas MD Anderson Cancer Center,...
Highlights • INs-seq: a new technology for recording scRNA-seq and intracellular protein activity • INs-seq defines new immune subsets by TF combinations and metabolic activity • Mapping Arg1+ cells within tumors reveals novel Trem2+ suppressive myeloid cells • Genetic ablation of Trem2 decreases Mreg, exhausted CD8+ T cells and tumor growth Summary Cell function and activity are regulated through integration of signaling, epigenetic, transcriptional, and metabolic pathways.
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