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Abstract The mammalian brain consists of diverse neuron types with various functions. Recent single-cell RNA sequencing approaches have led to a whole-brain taxonomy of transcriptomically defined cell types1. Patch-seq experiments augment these cell-type descriptions by linking transcriptomic profiles with local morphological and electrophysiological properties2,3,4,5,6,7. However, linking transcriptomic identities to long-range axonal projections remains a major unresolved challenge.
Keywords epigenetics transcriptomics enhancer cell type neocortex AAV transgenic recombinase mouse human Introduction To understand how the brain works, we need to establish the structure-function relationships between the units that comprise it and the outputs of its activity.1 One approach is to gain experimental access to each building block (e.g., a cell type) and manipulate it to observe effects on an animal’s physiology and behavior.2,3,4 Historically, the definition of building blocks...
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