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Abstract We present Ultra-Mild Bisulfite Sequencing (UMBS-seq), a method for 5-methylcytosine (5mC) detection that minimizes DNA degradation and background noise. UMBS-seq outperforms conventional bisulfite and enzymatic methyl-sequencing (EM-seq) methods in library yield, complexity, and conversion efficiency when applied to low-input DNA samples.
ArticleOnline now102324Open access 1Department of Medicine, The University of Chicago, Chicago, IL 60637, USA 2Department of Obstetrics & Gynecology, The University of Chicago, Chicago, IL 60637, USA 3Department of Chemistry, The University of Chicago, Chicago, IL 60637, USA 4Pritzker School of Molecular Engineering, The University of Chicago, Chicago, IL 60637, USA 5Health Science, DePaul University, Chicago, IL 60614, USA 6Department of Radiology and Imaging Sciences, Emory University...
Abstract Circulating cell-free RNA (cfRNA) in plasma represents a promising avenue for cancer detection. We report low-input multiple methylation sequencing, a method for profiling modification patterns in cfRNA, enabling the detection of diverse transfer RNAs and small noncoding RNAs derived from both the human genome and the microbiome.
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