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Articles by Zemer Gitai
CBASS limits bacteriophage production while maintaining cell viability in Pseudomonas aeruginosa
Keywords CBASS CapV anti-phage immunity cell viability Pseudomonas aeruginosa 3′,3′-cGAMP abortive infection Get full text access Log in, subscribe or purchase for full access. References 1. Li, Y. ∙ Slavik, K.M. ∙ Toyoda, H.C. ... cGLRs are a diverse family of pattern recognition receptors in innate immunity Cell. 2023; 186:3261-3276.e20 2. Slavik, K.M. ∙ Morehouse, B.R. ∙ Ragucci, A.E. ... cGAS-like receptors sense RNA and control 3′2′-cGAMP signalling in Drosophila Nature. 2021; 597:109-113 3. Cai, H.
Prophages block cell surface receptors to preserve their viral progeny - Nature
Abstract In microbial communities, viruses compete for host cells and have evolved diverse mechanisms to inhibit competitors. One strategy is superinfection exclusion, whereby an established viral infection prevents a secondary infection of the same cell1. This phenomenon has been shown to have an important role in the spread of eukaryotic viruses.
C. elegans transgenerational avoidance of P. fluorescens is mediated by the Pfs1 sRNA and vab-1
Abstract In its natural habitat, Caenorhabditis elegans must distinguish friend from foe. Pseudomonas are abundant in the worm’s environment and can be nutritious or pathogenic. Previously, we found that worms learn to avoid Pseudomonas aeruginosa and Pseudomonas vranovensis through a small RNA (sRNA)–mediated pathway targeting the C. elegans gene maco-1, and this behavior is inherited for four generations. Here, we show that C.
Sensing the shape of a surface by intracellular filaments
Abstract Understanding the mechanisms that dictate the localization of cytoskeletal filaments is crucial for elucidating cell shape regulation in prokaryotes. The actin homolog MreB plays a pivotal role in maintaining the shape of many rod-shaped bacteria such as Escherichia coli by directing cell-wall synthesis according to local curvature cues. However, the basis of MreB's curvature-dependent localization has remained elusive.
Global protein turnover quantification in Escherichia coli reveals cytoplasmic recycling under nitrogen limitation - Nature Communications
Abstract Protein turnover is critical for proteostasis, but turnover quantification is challenging, and even in well-studied E. coli, proteome-wide measurements remain scarce. Here, we quantify the turnover rates of ~3200 E. coli proteins under 13 conditions by combining heavy isotope labeling with complement reporter ion quantification and find that cytoplasmic proteins are recycled when nitrogen is limited.
Free-floating Bacteria Transcriptionally Respond to Shear Flow
Abstract Planktonic (free-floating) cells are typically assumed to be oblivious to any flow that carries them. Here we discover that planktonic bacteria can sense flow to induce gene expression changes that are beneficial in flow. Specifically, planktonic P. aeruginosa induce shear-rate-dependent genes that promote growth in low oxygen environments. Untangling this mechanism revealed that in flow, motile P.
P. aeruginosa controls both C. elegans attraction and pathogenesis by regulating nitrogen assimilation
Abstract Detecting chemical signals is important for identifying food sources and avoiding harmful agents. Like most animals, C. elegans use olfaction to chemotax towards their main food source, bacteria. However, little is known about the bacterial compounds governing C. elegans attraction to bacteria and the physiological importance of these compounds to bacteria.
Type-IV pili tune an adhesion-migration trade-off during surface colonization of Pseudomonas aeruginosa
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C. elegans interprets bacterial non-coding RNAs to learn pathogenic avoidance
Abstract Caenorhabditis elegans must distinguish pathogens from nutritious food sources among the many bacteria to which it is exposed in its environment1. Here we show that a single exposure to purified small RNAs isolated from pathogenic Pseudomonas aeruginosa (PA14) is sufficient to induce pathogen avoidance in the treated worms and in four subsequent generations of progeny.
A Dual-Mechanism Antibiotic Kills Gram-Negative Bacteria and Avoids Drug Resistance
Highlights • SCH-79797 kills Gram-negative and Gram-positive bacteria with undetectable resistance • It works by simultaneously targeting folate metabolism and membrane integrity • SCH’s dual-targeting is synergistic, but only when on the same chemical scaffold • Irresistin-16, an SCH derivative, effectively treats mouse N. gonorrhoeae infection Summary The rise of antibiotic resistance and declining discovery of new antibiotics has created a global health crisis.
Surface association sensitizes Pseudomonas aeruginosa to quorum sensing
Abstract In the pathogen Pseudomonas aeruginosa, LasR is a quorum sensing (QS) master regulator that senses the concentration of secreted autoinducers as a proxy for bacterial cell density. Counterintuitively, previous studies showed that saturating amounts of the LasR ligand, 3OC12-HSL, fail to induce the full LasR regulon in low-density liquid cultures. Here we demonstrate that surface association, which is necessary for many of the same group behaviors as QS, promotes stronger QS responses.
How to Build a Bacterial Cell: MreB as the Foreman of E. coli Construction
Cell shape matters across the kingdoms of life, and cells have the remarkable capacity to define and maintain specific shapes and sizes. But how are the shapes of micron-sized cells determined from the coordinated activities of nanometer-sized proteins? Here, we review general principles that have surfaced through the study of rod-shaped bacterial growth. Imaging approaches have revealed that polymers of the actin homolog MreB play a central role.
The effect of antibiotics on protein diffusion in the Escherichia coli cytoplasmic membrane
Escherichia coli strain VS116 (?flhC derived from the K12 strain RP437 [ 15 ]) was used for FRAP experiments without vancomycin treatment. A porous membrane E. coli strain with the imp4213 mutation, derived from FB83 background [ 16 ], was used for vancomycin experiments.
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