Zhuying Wei

Abstract The derivation of authentic embryonic stem cells (ESCs) across mammalian species remains a major challenge. Here, we report the development of a defined, serum-free culture system, termed 6iL/E4, that enables the derivation and long-term self-renewal of ESCs across diverse mammalian species. Through systematic dissection of signaling pathways, we identified conserved regulatory modules involving GSK3α, WNT, STAT3, PDGFR, and MEK/ERK signaling.

Correction to: Nature Biotechnology https://doi.org/10.1038/s41587-025-02832-4, published online 7 October 2025. In the version of this article initially published, due to a figure preparation mistake, the bottom-right immunohistochemistry images in Fig. 3d presented a sheep–sheep chimera, whereas it should have shown a cow–cow chimera, as now appears in the HTML and PDF versions of the article. For comparison, the original Fig.

Dear Editor, Utilizing CRISPR/Cas tools has simplified the creation of genome-edited cells, but the rapid generation of precisely edited animals is challenging, especially in domestic livestock. The widely used Cas endonuclease is Streptococcus pyogenes Cas9 (SpCas9, ~1368 amino acids (aa)), which creates double-strand breaks (DSBs) in target DNA sequences1. Then, the DSB is repaired through homology-directed repair (HDR) using a template.