Qi-Long Ying

Abstract The derivation of authentic embryonic stem cells (ESCs) across mammalian species remains a major challenge. Here, we report the development of a defined, serum-free culture system, termed 6iL/E4, that enables the derivation and long-term self-renewal of ESCs across diverse mammalian species. Through systematic dissection of signaling pathways, we identified conserved regulatory modules involving GSK3α, WNT, STAT3, PDGFR, and MEK/ERK signaling.

Results Overcoming key bottlenecks in engineered myeloid cell therapy requires a scalable source of myeloid progenitors. We developed a chemically defined culture formulation, termed SCF/2i, composed of stem cell factor (SCF), GDC-0879 (BRAF inhibitor),18 and SKL2001 (a reported Wnt/β-catenin modulator),19 that enabled log-phase expansion of primary mouse bone marrow cells for more than 50 days (Figure 1A; see STAR Methods).

Results Overcoming key bottlenecks in engineered myeloid cell therapy requires a scalable source of myeloid progenitors. We developed a chemically defined culture formulation, termed SCF/2i, composed of stem cell factor (SCF), GDC-0879 (BRAF inhibitor),18 and SKL2001 (a reported Wnt/β-catenin modulator),19 that enabled log-phase expansion of primary mouse bone marrow cells for more than 50 days (Figure 1A; see STAR Methods).