Qi-Long Ying

Correction to: Nature Biotechnology https://doi.org/10.1038/s41587-025-02833-3, published online 30 September 2025. In the version of this article initially published, the “FPC” image in Extended Data Fig. 2d was inadvertently duplicated from the “+CP-67” image in Extended Data Fig. 3k during figure assembly. Extended Data Fig. 2d has been updated with the intended FPC image in the HTML and PDF versions of the article.

Abstract The derivation of authentic embryonic stem cells (ESCs) across mammalian species remains a major challenge. Here, we report the development of a defined, serum-free culture system, termed 6iL/E4, that enables the derivation and long-term self-renewal of ESCs across diverse mammalian species. Through systematic dissection of signaling pathways, we identified conserved regulatory modules involving GSK3α, WNT, STAT3, PDGFR, and MEK/ERK signaling.

Results Overcoming key bottlenecks in engineered myeloid cell therapy requires a scalable source of myeloid progenitors. We developed a chemically defined culture formulation, termed SCF/2i, composed of stem cell factor (SCF), GDC-0879 (BRAF inhibitor),18 and SKL2001 (a reported Wnt/β-catenin modulator),19 that enabled log-phase expansion of primary mouse bone marrow cells for more than 50 days (Figure 1A; see STAR Methods).